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KMID : 1007520120210020607
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Food Science and Biotechnology
2012 Volume.21 No. 2 p.607 ~ p.611
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Antioxidant and Prooxidant Activities of β-Carotene in Accelerated Autoxidation and Photosensitized Model Systems
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Ha Dong-Oh
Park Chan-Uk
Kim Mi-Ja
Lee Jae-Hwan
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Abstract
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Antioxidant or prooxidant activity of β-carotene was studied in autoxidized or methylene blue photosensitized lard using the depletion of headspace oxygen, p-anisidine value (p-AV), or a modified DPPH methods. β-Carotene of 1.25 and 2.50 μM significantly decreased headspace oxygen and β-carotene from 0.25 to 2.50 μM increased significantly p-AV in lard oxidized at 60oC for 60 h (p<0.05), implying β-carotene acted prooxidantly. β-Carotene under 0.25 μM in oxidized lard did not decrease the absorbance of DPPH significantly (p>0.05), while 1.25 and 2.50 μM β-carotene decreased the absorbance of DPPH significantly (p<0.05), indicating β-carotene over 1.25 μM accelerated the formation of radical scavenging compounds from oxidized lipids (RSOLs). However, all the concentration of β-carotene did not cause significant changes in DPPH absorbance and headspace oxygen content (p>0.05) in photosensitized lard at 40oC for 24 h, indicating antioxidant properties of β- carotene in photosensitization.
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KEYWORD
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β-carotene, DPPH, photosensitization, lipid oxidation, accelerated autoxidation
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